Journal: Open Biology

Article Title: Acquisition of the dorsal structures in chordate amphioxus

doi: 10.1098/rsob.160062

Figure Lengend Snippet: Distribution pattern of active Arp2/3 complex in unfertilized and fertilized egg and its co-localization with microtubules and actin filaments. Partial rendering lateral images near maximum diameter in all but ( d (iv­–vi)), which are viewed vegetally near vegetal pole. ( a ) Eggs are oriented by the aid of polar body (pb) and/or vegetal tuft-like structure (tu). ( b (i–vi)) Change in location of cortical pArp2 immunopositive signals after sperm fusion from ubiquitous in cortical region in unfertilized egg ( b (i)) to on the one side of egg ( b (vi)) passing through animal pole ( b (iii,iv)) (arrowheads). ( c (i–iii)) Co-localization of F-actin and pArp2 immunopositive signals in late fertilized egg. ( d (i­–vi)) Co-localization of microtubules and pArp2 immunopositive signals in late fertilized egg. Note a tuft-like structure at the vegetal pole in immunostain for pArp2 and for tubulin ( d (ii)). Scale bar, 100 µm.

Article Snippet: The antibodies were anti-Arp2 (Thr237/Thr238) phospho-specific antibody (AP3871, ECM Biosciences, KY) for detecting active Arp 2/3 complex and anti-phospho-Smad1 (Ser463/465) antibody (06-702, Merck Millipore, Germany) for Bmp signalling.

Techniques:

Journal: Open Biology

Article Title: Acquisition of the dorsal structures in chordate amphioxus

doi: 10.1098/rsob.160062

Figure Lengend Snippet: Distribution patterns of maternal nodal mRNA and Arp2/3 complex. ( a (i) –h (i)) Maternal nodal mRNA (red) and pArp2 immunopositive signals (green) are co-localized from one-cell to blastula stage shown as partial rendering images near maximum diameter. Animal pole to the top in ( a (i)), ( d (i)) and ( h (i)). ( a (ii) –h (ii)) Relative fluorescent intensity curve at section denoted by white arrow that indicates direction of x -axis. Red for nodal and green for pArp2. Note a tuft-like immunopositive for anti-pArp2 antibody in a cell at 16- and 64-cell stage (arrowheads) ( e (i), g (i)). ( i (i–iii)) Expression pattern of nodal in CK666-treated blastula to late gastrula stage. ( j (i–iii)) Expression pattern of lefty in CK666-treated blastula to late gastrula stage. Note expression at median furrow (arrowheads) and a half of embryo in both genes. ( k (i–iii)) Co-localization of pArp2 immunopositive signals and nodal mRNA in CK666-treated blastula. Bl, blastula; bp, blastopore; iG, initial gastrula; lG, late gastrula. Scale bar, 100 µm.

Article Snippet: The antibodies were anti-Arp2 (Thr237/Thr238) phospho-specific antibody (AP3871, ECM Biosciences, KY) for detecting active Arp 2/3 complex and anti-phospho-Smad1 (Ser463/465) antibody (06-702, Merck Millipore, Germany) for Bmp signalling.

Techniques: Expressing

Journal: American Journal of Cancer Research

Article Title: Antineoplastic efficacy profiles of avapritinib and nintedanib in KIT D816V + systemic mastocytosis: a preclinical study

doi:

Figure Lengend Snippet: Specification of monoclonal antibodies (mAb)

Article Snippet: EMR2 , CD312 , REA302 , mouse , IgG1 , PE , Miltenyi Biotec.

Techniques: Bioprocessing, Control